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Asian Journal of Animal and Veterinary Advances

Year: 2011  |  Volume: 6  |  Issue: 4  |  Page No.: 379 - 384

Differential Gene Expression in Different Tissues of Black-Bone Sheep and Normal Sheep

Y. Chen, S.L. Yang, W.D. Deng and H.M. Mao

Abstract

In order to investigate the molecular mechanism of black-traits in the black-bone sheep, differential mRNA fingerprinting by prerential coding sequences amplification method was used to analyze the differential gene expression in live, heart, pancrease and lumbar muscle between the black-bone sheep and the Lanping native sheep, respectively. Seventy-one expressive sequence tags were obtained between the black-bone sheep and the Lanping native sheep. The results showed that the black-trait of black-bone sheep was controlled by major genes, as well as minor genes based on many differential expressive fragments. and also showed among positive expressive sequence tags in the black-bone sheep, one EST had high similarity (99%) to gluttathion-s-transferase of human (GSTs).

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