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Asian Journal of Animal and Veterinary Advances
Year: 2011  |  Volume: 6  |  Issue: 12  |  Page No.: 1193 - 1203

Bone Oxidative Changes during Early Fracture Healing of Postmenopausal Osteoporosis Rat Model

Ahmad Nazrun Shuid, Sharlina Mohamad, Norazlina Mohamed, Sabarul Afian Mokhtar, Norliza Muhammad and Ima Nirwana Soelaiman    

Abstract: Ovariectomised rat is an accepted model of postmenopausal osteoporosis. Fracture can be induced in this model to simulate osteoporotic fracture. The aim of the study was to determine the anti-oxidative changes during early fracture healing in postmenopausal osteoporosis rat model. The lipid peroxidation levels and anti-oxidant enzyme activities during the early phase of osteoporotic fracture healing were measured directly in the fractured bone of ovariectomised rats. The effects of antioxidant supplementation i.e., vitamin E, on the oxidative parameters were also measured. Thirty two female Sprague-Dawley rats were divided into groups of Sham-Operated (SO), Ovariectomised-Control (OVC), ovariectomised +60 mg kg-1 of α-tocopherol (ATF) and ovariectomised +60 mg kg-1 of Tocotrienol-rich fraction (TT). The rats were left untreated for 2 months to allow ovariectomy-induced osteoporosis before their right femora were fractured at the mid-diaphysis. After 2 weeks of treatment, the Thiobarbituric Acid Reactive Substances (TBARS) level and Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPX) enzyme activities of the fractured femora were measured. There was a significant drop in the SOD activity of OVC group compared to SO group. Tocotrienol was able to significantly lower the TBARS level and raise the GPX activity compared to OVC group. While, α-tocopherol was able to significantly raise the SOD activity compared to OVC group. Therefore, only SOD activity was affected during early fracture healing in this rat model. Tocotrienol was able to reduce lipid peroxidation during early fracture healing of osteoporotic bone probably by enhancing its GPX activity. Anti-oxidants were beneficial in improving the bone oxidative status during early fracture healing.

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