Positive Response of Isolated Rat Pancreatic Islets to IMOD; Hopes for Better Transplant Outcome and Graft Function
IMOD as a multi-herbal-selenium electromagnetically-treated combination was tested for its potential on the function and level of Reactive Oxygen Species (ROS) in isolated rat pancreatic islets. After Laparotomy, pancreas were removed and islets were isolated and incubated in RPMI 1640 for 24 h and then islets were separated and divided into several tubes containing ten in each. IMOD at logarithmic doses (0.1, 1, 10, 100 and 1000 ppm) were added to islets and incubated for 24 h and then static insulin secretion, at low dose (2.8 mM) glucose and high dose (16.7 mM) glucose was tested. Cells viability and ROS were also determined. IMOD at 0.1 ppm increased islet insulin secretion in response to glucose in both basic and stimulation levels. This drug at 1 ppm dose only increased insulin concentration in stimulation levels of glucose. IMOD at 10, 100 and 1000 ppm decreased insulin secretion in both levels of glucose. The viability at the doses of 0.1 and 1 ppm increased. The result of ROS test showed a decrease although significant, at the lower dose (0.1 μM) but increased dose-dependently at the doses of 10, 100 and 1000 ppm. IMOD has significant anti oxidative effects at low doses and improves viability and insulin secretion of isolated islets in both basic and stimulation levels of glucose. Pretreatment with IMOD may improve transplant outcome and graft function. Its effect in insulin release maybe promising in treatment of diabetes.
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