PCR-Based Detection of Yersinia ruckeri Infection in Rainbow Trout Fish
A. Haghighi- Khiabanian-Asl,
The aim of this study was designing a diagnostic kit
for yersiniosis in the trout fish in Iran. Colonies of Yersinia ruckeri
were collected from culture medium and a suspension was prepared in a
lysing solution. DNA was extracted through a boiling and phenol chloroform
method. Two primer sets targeting bacterial 16S rRNA and trout 18S rRNA.
Polymerase chain reaction products were separated by gel electrophoresis.
Among 20 suspected samples tested two samples were positive for both host
and bacterial PCRs indicating the positive Y. ruckeri infection
and remaining 18 samples were negative for pathogen. The performance of
PCR reactions in negative samples were confirmed from amplification of
internal control reactions targeting host. A PCR based diagnostic kit
with an internal control was prepared for detection of Yersinia ruckeri
in rainbow trout fish.
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