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Asian Journal of Animal and Veterinary Advances

Year: 2008  |  Volume: 3  |  Issue: 6  |  Page No.: 400 - 408

Effect of Epididymis Handling Conditions on the Quality of Ram Spermatozoa Recovered Post-Mortem

M. Shakeri, H. Roshanfekr, M. Mamoei and Kh. Mirzadeh


The present research studied the effect of the interval between animal`s death and sperm recovery (0, 24 or 48h) on the quality and freezability of ram spermatozoa from cauda epididymidis. Storage temperature of epididymes (room temperature or 5°C) was also analyzed. Spermatozoa were diluted with Tes-Tris-Fructose solution supplemented with egg yolk (10%) and glycerol (4%) and frozen using a programmable bio-freezer (-20°C min-1). Pre-freeze and post-thaw sperm samples showed viable spermatozoa up to 48h after the animal`s death, although their quality declined significantly as post-mortem storage time increased. Epididymes sperm stored at 5°C showed better motility and a lower percentage of abnormal forms than epididymes stored at room temperature after 24 and 48h. The fertilizing ability of cauda epididymis ram spermatozoa obtained at 0 and 24h after the animal`s death is similar to that of ejaculated spermatozoa. Therefore, a good protocol for post-mortem semen collection in rams when epididymal spermatozoa cannot be collected immediately is to preserve the epididymes at 5°C and process the samples in the first 24h after the animal`s death.

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