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Asian Journal of Animal and Veterinary Advances
Year: 2008  |  Volume: 3  |  Issue: 5  |  Page No.: 375 - 380

The Effects of Combination of Citric Acid and Microbial Phytase on the Concentration of Some Minerals of Serum and Parameters of Mineralization of Tibia in Commercial Laying Hens

Yahya Ebrahim Nezhad, Naser Maheri Sis, Abolfazl Aghajanzadeh Gholshani, Yaghob Saedi and Reza Aminvakili    

Abstract: This experiment was conducted to evaluate the combined effect of citric acid and microbial phytase on the concentration of minerals of serum and parameters of mineralization of tibia in Hy-line commercial layers (W-36) in 53-64 weeks of age. One hundred and ninety two of laying hens were tested. The experimental design was completely randomized design with a 3x2 factorial arrangement with three levels (0, 2 and 4%) of citric acid and two levels (0.0 and 300 FTU kg-1) of microbial phytase in low available phosphorus diets with 6 treatments, 4 replicates and 8 hens in each replicate. The concentration of zinc, copper and manganese of serum and ash, calcium and phosphorus of tibia was evaluated. The results showed that interaction between citric acid and microbial phytase significantly affected the concentration of copper in serum, tibia ash and tibia ash calcium percentage (p < 0.01). Using 300 FTU kg-1 microbial phytase into low available phosphorus diets increased tibia ash phosphorus percentage (p < 0.05).

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