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Asian Journal of Animal Sciences
Year: 2017  |  Volume: 11  |  Issue: 3  |  Page No.: 132 - 139

Molecular Detection of Polymorphism of Heat Shock Protein 70 (Hsp70) in the Semen of Iraqi Holstein Bulls

Hassan Nima Habib, Amad Falah Hassan and Bassam Yasein Khudaier    

Abstract: Background and Objective: Hsp70 is a molecular chaperone and cell preservation against heat shock, considered as the ideal biological marker for measuring heat stress in animals, its polymorphism may affect stress tolerance bulls’ fertility. The study aimed to identify polymorphism of hsp70 gene in Holstein bulls born in Iraq. Materials and Methods: The study was carried out during 1st November, 2015 to 31th January, 2016, 29 Holstein bulls born in Iraq, 2.5-3.5 years old, back to Artificial Insemination Center of Abou-Ghareeb Baghdad, Iraq, were used. Semen was collected by using the artificial vagina. DNA extracted then PCR amplified, sequencing, BLAST analysis and multiple sequence alignment were carried out. Results: The results showed three haplotypes, haplotype G1, haplotype G2 and haplotype G3 as a compare with the same gene in GenBank, they all showed silent mutations in different positions except haplotype G2 showed a missense mutation in position 1451 as well as silent mutations. Conclusion: It is concluded that the Holstein bulls born in Iraq have new polymorphism of Hsp70 gene as a compare with same gene in GenBank, because of mutations.

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