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Asian Journal of Animal Sciences
Year: 2016  |  Volume: 10  |  Issue: 1  |  Page No.: 92 - 98

Analysis of Seminal Plasma Proteins of South Indian Jersey and Hybrid Bulls and their Correlation with Semen Quality

A.S. Vickram, V. Devi Rajeswari, M. Ramesh Pathy and T.B. Sridharan    

Abstract: This is a preliminary study of the protein profiles of the semen in Jersey breed bulls. Jersey bulls were mainly used for fertility purposes; they were grouped based on the fertility, such as highly fertile group (95% fertility), medium fertile group (65-70%), low fertile group (45-50%) and hybrid bulls. Semen of 20 Jersey and hybrid bulls was collected through artificial vagina. The semen characteristics includes volume, pH value, viscosity, viability, sperm concentration, agglutination, total motility and their group, sperm morphology and Hypo-Osmotic Swelling test (HOS) were carried out immediately after collection. Total antioxidant capacity test by using catalase and cholesterol analysis were also carried out. Quantification of the amino acids and proteins were carried out by Ninhydrin and Lowry method, protein analysis by Sodium Dodecyl Sulphate (SDS) was carried out for the following categories. (1) Highly fertile group (Jersey), (2) Medium fertile group (Jersey), (3) Low fertile group (Jersey) and (4) Hybrid group. Analysis of semen protein reveals about seventeen protein bands of sizes ranging between 14 and 205 kDa were identified. Among those proteins bands, the relative protein content of nine bands shows significantly different from each group. The rest of the proteins bands seem to have some positive and/or negative correlation on the semen characterizes or fertility. The antioxidant capacity of highly fertile and hybrid group was very high compare to low fertile group of Jersey. This study clearly indicates the bull seminal plasma proteins are associated with fertility and as well as determination of semen quality and ultimately decides the fertility.

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