Identification of Single Nucleotide Polymorphisms and Allele Distribution of MC1R Gene in Different Head and Neck Color of Ettawa Grade Goat
I Gede Suparta Budisatria
Objective: The melanocortin-1-receptor (MC1R) gene is known as an important candidate gene for the coat color trait. The present study identified the polymorphisms and tested whether a functional polymorphism in exon 1 of MC1R gene was related to different head and neck color in Ettawa grade goat based on their allele distribution. Methodology: Total 30 Ettawa grade goats were divided in three group, CP: Brown head and neck color with white body color, RP: Brown or black head and neck color with various body color and HP: Black head and neck color with white body color were used in this study. The blood samples from all groups were collected for DNA isolation. Some samples were used to identify the polymorphisms by direct sequencing. Three Single Nucleotide Polymorphisms (SNPs) of MC1R gene were identified: SNP 676A>G, SNP 748T>G and SNP 801C>G. The SNP 676A<G was performed for genotyping by using EarI restriction enzyme. Results: The A and G allele frequencies were similar 50% in both CP and RP group. However, the A allele frequency (55%) was slightly higher than G allele (45%) in HP group. The A and G allele distribution in all the group population was nearly the same (X2 = 0.016, df = 1, p = 0.898). All of the goats in CP and RP groups had heterozygote (AG) genotype. In HP group only had one goat with homozygote animal (GG genotype). Interestingly, none AA genotype found in this study. Conclusion: In conclusion, the allele distributions were equal in all groups and almost all the goats with different head and neck color have similar heterozygote genotype.
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