Effect of Antioxidants on the Stored Dromedary Camel Epididymal Sperm Characteristics
I.M. Abd El-Razek,
This study was conducted to determine the effect of different type and levels of antioxidant supplementation (0.4 and 0.8 mM from glutathione, GSH or 0.5 and 1.0 g L1 extender from ascorbic acid, AA) as compared to control, on characteristics of camel epididymal spermatozoa stored at 25°C (room temperature) for 0, 2, 4 and 12 h or at 5°C (cool temperature) for 0, 12, 24 and 48 h. Testis of camel were collected after animal slaughtering and placed immediately into plastic bag into ice box at 5°C. Epididymal spermatozoa were collected by aspiration from tail and extended with tris-egg yolk extender. Results of epididymal spermatozoa stored at 25°C showed improvement in livability (p<0.05) and abnormality (p≥0.05) with GSH (0.4 mM), while sperm motility and curling spermatozoa improved (p≥0.05) with AA (0.5 g L1). Storage at 5°C improved (p<0.05) motility, livability and curling spermatozoa with GSH (0.4 mM), while sperm abnormality improved (p<0.05) with AA (1 g L1). At different incubation times at 25 or 5°C, percentages of motility, livability and curling spermatozoa decreased (p<0.05) and of sperm abnormality increased (p<0.05) by increasing storage time. The effect of interaction between antioxidant supplementation and storage time on all sperm characteristics studied was not significant. In conclusion, supplementation of tris-egg yolk extender with GSH (0.4 mM) or AA (0.5 g L1) has a vital role in maintaining function, morphology and membrane integrity of epididymal camel spermatozoa stored at 25°C for 12 h or at 5°C for 48 h, respectively.
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