Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Asian Journal of Agricultural Research
Year: 2016  |  Volume: 10  |  Issue: 2  |  Page No.: 126 - 131

Minimal Inhibitory Concentration of Common Selectable Agents on Recalcitrant Malaysian Rice Cultivar

Clement Wong Kiing Fook, Lai Kok Song, Wong Mui Yun and Maziah Mahmood    

Abstract: Determining the suitable and optimized Minimal Inhibitory Concentration (MIC) of the selectable agents is critical to successfully recover stably transformed plants. In this study, the MIC of five different antibiotics and one herbicide was determined for their inhibitory effect on the growth of a recalcitrant rice cultivar, MR219 using rice shoot apices as target tissue. Antibiotics such as kanamycin, paromomycin and neomycin did not inhibit the shoot apices growth while geneticin (G–418) and hygromycin showed complete growth inhibition at 400 and 25 mg L–1, respectively. Similarly, the herbicide basta indicated that 9 mg L–1 was sufficient to cause growth inhibition. To conclude, hygromycin and basta were suggested to the suitable selectable agent to select transformed rice shoot apices.

Cited References   |    Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility