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Agricultural Journal
Year: 2013  |  Volume: 8  |  Issue: 2  |  Page No.: 60 - 65

Genetic Characterization of Canine Parvovirus Isolates From Beijing of China Between 2009 and 2011

Jing Wang, Li-Jun Shi, Peng Wang, Zhan-Zhong Zhao, Miao-Miao Gong, Gang Li, Wei-Feng Yuan and Hong-Fei Zhu    

Abstract: In order to investigate the new strains circulating in Beijing, 24 positive samples of CPVs were isolated from Beijing of China between 2009 and 2011 and the sequences of the main capsid protein VP2 were obtained. These sequences were analyzed in comparison with the sequences of 29 other strains of CPV from GenBank. Two types of CPV, including CPV-2 and CPV-2a were detected and CPV-2a (with Ala297 mutation) was predominant in Beijing and 4 strains CPV-2a types were found to carry the amino acid substitution Ala440. The unique Ile324 mutation in the VP2 of Beijing CPV isolate was detected as compared with a Tyr324 in the VP2 of the reference CPV strains. A phylogenetic tree was constructed from the VP2 genes. These results showed that most of the isolated Beijing strains classified in a cluster Chinese field isolates which were distinct from American and Italian isolates.

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