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Agricultural Journal
Year: 2011  |  Volume: 6  |  Issue: 1  |  Page No.: 31 - 34

Detection of Streptococcus agalactiae Existence within Milk Samples of Hair Goats Grown in West Anatolia Region

Dilek Keskin, Okan Atay, Sukru Kirkan, Ozdal Gokdal, Serten Tekbiyik and Osman Kaya    

Abstract: This study is performed to identify existence of Streptococcus agalactiae being mastitis factor in hair goats grown in Cine district of Aydin province in West Anatolia region. Material of the study consists of samples of 232 goat milk. Samples have been received from 116 goats of 9 herds grown in Kavsit, Tatarmemisler, Catak, Ibrahimkavagi towns of Cine district. Samples have been delivered to routine diagnosis laboratory of Adnan Menderes University, Faculty of Veterinary Science, Microbiology Departments to analyze them under cold chain and in terms of S. agalactiae. For S. agalactiae isolation purposes, double inoculation of sheep blood agar of each milk sample to be analyzed has been applied. Inoculated milk samples have been left for inoculation during 24-48 h at 37°C in both aerobic and micro aerobic medium. After incubation, gram staining has been applied to milk samples shaped, gram positive cocci have been sorted and catalyze test has been applied to gram positive cocci. Catalyze negative colonies have been separated to which other chemical tests have been applied. At the end of analysis, S. agalactiae has been identified in 12 of 116 goats samples (10.3%).

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