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Acta Biochimica et Biophysica Sinica
Year: 2010  |  Volume: 42  |  Issue: 12  |  Page No.: 893 - 899

Beneficial effects of C-peptide on renal morphology in diabetic rats

W Sun, X Gao, X Zhao, D Cui and Q. Xia    


The study was undertaken to examine the effects of C-peptide on glomerular volume (VGLOM), mesangial matrix synthesis, and degradation in streptozotocin (STZ)-diabetic rats with poor or moderate glycemic control. Series 1 (poor glycemic control) included groups of healthy rats, hyperglycemic rats, diabetic insulin-treated rats and diabetic C-peptide-treated rats. Series 2 (moderate glycemic control) included groups of healthy rats, diabetic insulin-treated rats, diabetic insulin- and C-peptide-treated rats. After 8 weeks, the left kidney was excised for evaluation of VGLOM and mesangial matrix area via light microscopy. Mesangial cells were cultured for 48 h and type IV collagen expression and matrix metalloproteinase (MMP)-2 expression were measured by ELISA and RT–PCR. The results indicated that in Series 1, C-peptide administration suppressed the diabetes-induced increase in the VGLOM and the mesangial matrix area. In Series 2, C-peptide administration resulted in a similar decrease in the VGLOM and a greater decrease in the mesangial matrix area when compared with insulin therapy alone. Moreover, C-peptide (300 nM) completely inhibited the glucose-induced increase of the collagen IV mRNA expression and protein concentration in mesangial cells cultured in 30 mM glucose medium. MMP-2 mRNA expression was not influenced by C-peptide. In conclusion, C-peptide administration to STZ-diabetic rats for 8 weeks results in the inhibition of diabetes-induced expansion of the mesangial matrix. This effect is independent of the level of glycemic control and results from the inhibition of diabetes-induced excessive formation of mesangial type IV collagen.

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