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Acta Biochimica et Biophysica Sinica

Year: 2010  |  Volume: 42  |  Issue: 9  |  Page No.: 623 - 627

Kinetic analysis of RNA interference for lamin A/C in HeLa cells

S. J You, S. H Lee and J. S. Lee

Abstract

We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug–response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.

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