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Turkish Journal of Veterinary and Animal Sciences
Year: 2009  |  Volume: 33  |  Issue: 5  |  Page No.: 379 - 384

Effects of addition of vitamin B12 to the extender on post-thaw motility, acrosome morphology, and plasma membrane integrity in bull semen

Jian-Hong HU, Qing-Wang LI, Yu-Lin CHEN, Zhong-Liang JIANG, Yong-Hong JIA, Li-Qiang WANG and Bo-Bo OU    

Abstract: To investigate the effects of supplementation of vitamin B12 on bovine sperm post-thaw quality, vitamin B12 was added at the concentrations of 1.25, 2.50, 3.75, and 5.00 mg/mL to bovine semen cryoprotective medium. The results indicated that the motility and VSL, VCL, STR, and VAP values of sperm supplemented with 2.50 mg/mL vitamin B12 were significantly higher than those of other concentrations (P < 0.05). No significant difference was observed for LIN and ALH values and the percentage of grade a spermatozoa between the extenders containing 2.50 mg/mL and 3.75 mg/mL vitamin B12 (P > 0.05). The percentages of acrosome-intact and plasma membrane-intact spermatozoa were significantly improved (P < 0.05) by supplementing with 2.50 mg/mL vitamin B12. With all parameters measured, the concentration of 2.50 mg/mL vitamin B12 showed the best results on the quality of bovine semen in freezing-thawing. In conclusion, the addition of 2.50 mg/mL vitamin B12 to the extender improved the bovine frozen semen quality.

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