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Insight Bacteriology
Year: 2013  |  Volume: 2  |  Issue: 1  |  Page No.: 3 - 9

Effect of Carbon and Nitrogen Sources of the Medium used to Grow Biocontrol Strain UTPF61 of Pseudomonas fluorescens on its Protective Activity Against Sclerotinia Wilt of Sunflower and its Survival During and after the Formulation Process

Fereshteh Heidari-Tajabadi, Masoud Ahmadzadeh, Vahid Fallahzadeh-Mamaghani and Asma Moeinzadeh    

Abstract: Background: Sclerotinia wilt caused by Sclerotinia sclerotiorum, is a devastating disease of sunflower worldwide. One promising area of disease management for resource-poor farmers that emerged in recent years in developing countries is the potential of biological control. Material and methods: Pseudomonas fluorescens strain UTPF61 is an effective biocontrol agent for sclerotinia wilt disease. One major constraint in large-scale field evaluation of biocontrol agents of sclerotinia wilt is their availability in large quantities. Fermentation is important in order to simplify production of biocontrol agents. But the large scale production of biocontrol agent is expensive because of the high cost of the medium. Then this problem is solved with the use of inexpensive agricultural byproducts such as molasses and corn steep. Results: This research investigated the effect of some combinations of carbon and nitrogen sources during fermentation of Pseudomonas fluorescens UTPF61 on its growth in flask and fermentor and its subsequent antagonistic activity in vitro and protective activity in plant. Its survival during the formulation process and shelf life over a period of 150 days was investigated. Rapid growth in flask was observed in medium containing Molasses+yeast extract but medium containing Molasses+2-ammonium hydrogen phosphate had the best effect on the antagonistic efficacy, biocontrol activity, PGPR and survival of bacteria.

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