Science Alert
Trends in Applied Sciences Research
  Year: 2011 | Volume: 6 | Issue: 6 | Page No.: 606-613
DOI: 10.3923/tasr.2011.606.613
Solubilization of Tricalcium Phosphate by Fungus Aspergillus niger at Different Carbon Source and Salinity
Janardan Yadav, Jay Prakash Verma and Kavindra Nath Tiwari

Plant Growth Promoting Fungus (PGPF) isolated from rhizosphere of chickpea and identified as Aspergillus niger strain BHUAS01 was tested for its tricalcium phosphate solubilizing ability at different sources of carbon viz., glucose, sucrose, glycerol and mannitol in Pikovskaya broth. Also, A. niger was analyzed solubilization of tricalcium phosphate (TCP) in broth media at different salinity viz., 1% NaCl, 1% KCl and 1% CaCl2 at varying range of reaction (pH) under in vitro condition. Among the carbon sources, Aspergillus niger was found to solubilize maximum tricalcium phosphate (512 μg mL-1) at glucose as carbon source and minimum activity (348 μg mL-1) of phosphate solubilization at sucrose as carbon. Aspergillus niger showed maximum significant solubilization of tricalcium phosphate in Pikovaskaya broth containing carbon source glucose followed by glycerol, maltose and sucrose at 21 days of incubation. Further the effect of different salinity (1% NaCl, 1% KCl and 1% CaCl2) was tested at different pH (6.0, 7.0 and 8.0) under in vitro condition. A. niger strain BHUAS01 was showed maximum significant solubilization of tricalcium phosphate (495 μg mL-1) in presence of 1% CaCl2 in modified Pikovskaya broth at pH 8.0 than other salt concentration. This finding can provide great benefit in the maintaining the available phosphates for crops in saline and alkaline soils. A large fraction of land arid and semiarid regions is affected by salinity in India.
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13 October, 2012
Sanjay Joshi:
I tried it.How it is possible,enzyme activity ends at 10 days only after wards it shows decrease in OD. Please clarify it.