Science Alert
Research Journal of Seed Science
  Year: 2012 | Volume: 5 | Issue: 3 | Page No.: 71-83
DOI: 10.3923/rjss.2012.71.83
Biochemical Changes Due to Seed Priming in Maize Hybrid COH(M) 5
S. Sathish, S. Sundareswaran, N. Senthil and K.N. Ganesan

Nowadays seed priming was proved to be an effective seed invigouration treatment. However, firm evidence on the molecular basis underlying priming treatments is either sparse or lacking. The objective of the present study was to ascertain the changes in seed protein profile viz., total, soluble and heat stable protein due to priming and the changes in DNA, phytate and minerals viz., B, Cu, Mg, Mn, Fe, Zn and K content immediately after priming and on subsequent germination of seeds of maize hybrid COH(M) 5. Seeds of maize hybrid were primed with water and 1% KH2PO4 for 6 h and the result revealed that seed priming with 1% KH2PO4 for 6 h can undoubtedly increase germination potential and vigour of maize hybrid COH(M) 5 and attributed reason was, increase in new protein synthesis, advancement of DNA replication and cell division, absorption of K ions due to priming with potassium salt and rapid utilization of food reserves like phytate, minerals viz., B, Cu, Mg, Mn, Fe Zn, K and imbibed K ions. The result of decrease in phytate content due to priming can be used in poultry sector to reduce the phytate content in poultry feed. However, further research is required to identify the specific proteins synthesised and characterization of molecular marker to standardise priming treatment for each batch of seed lots of maize hybrid COH(M) 5.
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26 June, 2012
Ajani, Olusola Oyebamiji:
This paper is very timely particularly for me,highly educative and informative about what happens to the seed during priming

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