Science Alert
FOLLOW US:     Facebook     Twitter
Research Journal of Phytochemistry
  Year: 2018 | Volume: 12 | Issue: 2 | Page No.: 60-70
DOI: 10.3923/rjphyto.2018.60.70
Metabolite Profiling of Crotalaria verrucosa Leaf Extract and Evaluation of its Antioxidant and Cytotoxic Potency
Zainab Syed Ahmed, Tasnova Nowrin, Md. Hemayet Hossain, Tanjima Nasrin and Raushanara Akter

Background and Objective: Crotalaria verrucosa (C. verrucosa) Fabaceae is a Bangladeshi medicinal plant, native to Sylhet and traditionally this plant is used as medicine for treating impetigo, scabies, salivation, jaundice, cough, biliousness, dyspepsia, fever, cardiac abnormalities and oral diseases. Since no previous study investigated its antioxidant or cytotoxic potential, the present study was aimed to analyze its antioxidant and cytotoxic potential for identification and quantification of those metabolites which contributed to these activities. Materials and Methods: Cold maceration using methanol was used for extraction from C. verrucosa leaves. Antioxidant activity was determined by total phenol content, total flavonoid content, total antioxidant capacity and DPPH free radical scavenging assays. Metabolite profiling was obtained by HPLC-DAD. Finally, cytotoxicity was evaluated using an MTT assay against HeLa cells. Results: The extract confirmed high phenolic content and high flavonoid content yet a moderate total antioxidant capacity and moderate inhibition (%) of DPPH free radical scavengers was found. Further HPLC analysis revealed the presence of 7 polyphenolic compounds from which gallic acid was predominantly present along with small quantities of (+) catechin hydrate, vanillic acid, caffeic acid, syringic acid, (-) Epicatechin and vanillin. Lastly, MTT assay evident the highest cell growth inhibition at a concentration of 2.5 mg mL1 and the IC50 value was found to be 0.83mg mL1 which indicated the strong cytotoxic potential of the extract. Conclusion: Strong cytotoxic potential and a moderate antioxidant potential were observed which justifies its role in folkloric remedies but extract needs further purification to use it as a folkloric remedy for oxidative stress-mediated diseases.
 [Abstract]   [Fulltext PDF]   [Fulltext HTML]   [References]   [View Citation]  [Report Citation]

20 November, 2018
Md. Awlad:
would be very helpfu for some fatal diseses.thanks all