Molecular Diagnosis of Naturally Infection with Eimeria nieschulzi in Laboratory Rats
Gautam Patra,
M. Ayub Ali,
Kh. Victoria Chanu,
Jonathan Lalsiamthara,
J.L. Kataria,
Snigdha Hazarika,
David Malsawmkima,
R. Ravindran and L. Inaotombi Devi
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Dr. Michael Kurth
I´ve some doubts that the identified species is Eimeria nieschulzi. We work for a long time with Eimeria nieschulzi and we never saw such necrotic tissue in the small intestine and never in the large intestine. Lesions caused by Eimeria nieschulzi are normally limited on the small intestine. Further, the shown oocyst in Figure 3 is (at least) not a (typical) Eimeria nieschulzi oocyst. The histological images have unfortunatly a too poor quality to see if the developmental stages are in the villi or in the crypts. With the used primer pair it is not possible to amplify the specific 18S rDNA of Eimeria nieschulzi. By the used primers you can amplify the 18S rDNA of every Eimeria species.The PCR reaction wasn´t a proof without sequencing.