Science Alert
Research Journal of Microbiology
  Year: 2011 | Volume: 6 | Issue: 2 | Page No.: 140-146
DOI: 10.3923/jm.2011.140.146
Characterization of Alpha Amylase from Bacillus subtilis BS5 Isolated from Amitermes evuncifer Silvestri
T.O. Femi-Ola and B.M. Olowe

Amylase producer Bacillus subtilis BS5 was obtained from the hindgut of wood eating termites Amitermis evuncifer Silvestri. Amylase enzyme produced by this strain when grown on basal liquid medium with cocoyam starch as substrate was characterized. The enzyme was purified through ion-exchange chromatography and gel-filtration. The purified enzyme had a molecular weight of 63 kDa and was active optimally at pH 6.0 and 50°C. The apparent Km value and Vmax of the enzyme during hydrolysis of soluble starch were 16.67 mg mL-1 and 3.8 mg/min/mL-1, respectively. The activities of the amylase were stimulated by Ca2+, Na+ and Mg2+ but inhibited by EDTA and HgCl2.
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09 March, 2011
tanya tejeswita:
very clean results