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| Variation in Germination and Growth Rates of Two Isolates of Beauvaria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes at Different Temperatures and their Virulence to Callosobruchus maculatus (Fabricius) (Coleoptera: Bruchidae) |
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Adaku A. Lawrence
and Ayub Khan
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Abstract: This study aims to determine the effect of temperature
on conidial germination, colony radial growth and virulence of two isolates
of Beauvaria bassiana (ARSEF-1186 and IMI-351833) to the cowpea
weevil, Callosobruchus maculatus under controlled laboratory conditions.
Isolates grown on Potato Dextrose Agar plates indicated that the rate
of conidial germination was slower at 20 °C than at 25 and 30 °C
and was expressed by the following 50% Germination Time (GT50)
values: 17.23 ± 1.02 h for ARSEF-1186 and 16.46 ± 1.02 h
for IMI-351833. The lowest GT50 value was estimated as 12.33
± 1.02 h for IMI-351833 at 25 °C. ARSEF-1186 showed significantly
faster colony radial growth rate (Kr) than IMI-351833 for all
temperatures investigated. Bioassays using Cajanus cajan seeds
dipped in conidial suspensions of both isolates indicated that the highest
virulence gainst C. maculatus adults was observed with ARSEF-1186
at 30 °C. This isolate exhibited both the lowest LC50 (2.29x105
spores mL-1) and LT50 (4.17 days) values. |
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| COMMENTS |
16 March, 2009
Jeff Lord:
Beauveria is spelled incorrectly in this paper. |
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