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| Micro-propagation of Damask Rose (Rosa damascena Mill.) cv. Almarah |
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T. Alsemaan
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Abstract: Almarah is the most important cultivar of Damask rose in Syria. This study
aimed to determine the best protocol for its in vitro micro-propagation.
The experiments were conducted at Damascus University in Syria. Effects of different
concentrations of benzyladenine BA (0, 0.5, 1 and 2 mg L-1) and gibberellic
acid GA3 (0, 1 and 2 mg L-1) were evaluated on proliferation of the
explants. To evaluate in vitro rooting, proliferated micro-shoots were
cultured on a MS/2 medium supplemented with 3 g L-1 activated charcoal
and (0, 1, 2 and 3 mg L-1) concentrations of Indol-3-Butyric Acid
IBA. The experimental design of the all stages was a Complete Randomized Design.
Means were analyzes with Duncan’s test at p≤0.05 using SPSS. BA increased
the proliferation of the explants up to 2 mg L-1. The interaction
of BA with GA3 significantly increased proliferation rate of the explants. The
highest number of proliferated explants was obtained in the presence of 2 and
2 mg L-1 of GA3 and BA, respectively. The highest percentage of rooted
explants was obtained in the presence of 3 mg L-1 IBA in the rooting
medium but the number and length of roots significantly increased in response
to increasing IBA concentration in the medium up to 2 mg L-1. Only
8% of the plantlets lost during hardening. For the first time in Syria a mass
in vitro production protocol of Syrian Damask rose has been achieved.
The interaction of BA (2 mg L-1) with (GA3 2 mg L-1) is
highly recommended for proliferation rate of the explants while adding IBA (2
mg L-1) to the MS/2 rooting medium is highly recommended for high
quality in vitro rooting. |
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| COMMENTS |
14 February, 2015
mojgan arefi:
hi.
plz give me article for tissu culture of rosa damascena.
thanks. |
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