Science Alert
International Journal of Agricultural Research
  Year: 2013 | Volume: 8 | Issue: 4 | Page No.: 172-177
DOI: 10.3923/ijar.2013.172.177
Micro-propagation of Damask Rose (Rosa damascena Mill.) cv. Almarah
T. Alsemaan

Almarah is the most important cultivar of Damask rose in Syria. This study aimed to determine the best protocol for its in vitro micro-propagation. The experiments were conducted at Damascus University in Syria. Effects of different concentrations of benzyladenine BA (0, 0.5, 1 and 2 mg L-1) and gibberellic acid GA3 (0, 1 and 2 mg L-1) were evaluated on proliferation of the explants. To evaluate in vitro rooting, proliferated micro-shoots were cultured on a MS/2 medium supplemented with 3 g L-1 activated charcoal and (0, 1, 2 and 3 mg L-1) concentrations of Indol-3-Butyric Acid IBA. The experimental design of the all stages was a Complete Randomized Design. Means were analyzes with Duncan’s test at p≤0.05 using SPSS. BA increased the proliferation of the explants up to 2 mg L-1. The interaction of BA with GA3 significantly increased proliferation rate of the explants. The highest number of proliferated explants was obtained in the presence of 2 and 2 mg L-1 of GA3 and BA, respectively. The highest percentage of rooted explants was obtained in the presence of 3 mg L-1 IBA in the rooting medium but the number and length of roots significantly increased in response to increasing IBA concentration in the medium up to 2 mg L-1. Only 8% of the plantlets lost during hardening. For the first time in Syria a mass in vitro production protocol of Syrian Damask rose has been achieved. The interaction of BA (2 mg L-1) with (GA3 2 mg L-1) is highly recommended for proliferation rate of the explants while adding IBA (2 mg L-1) to the MS/2 rooting medium is highly recommended for high quality in vitro rooting.
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14 February, 2015
mojgan arefi:
hi. plz give me article for tissu culture of rosa damascena. thanks.