Abstract: Indigofera trita L.F. SPP. subulata (vahl ex poir) distributed in the southern part of India particularly in Tamilnadu and it has potential medicinal properties and used in the treatment of tumours and liver disorders. The work carried out in the plant is much less, the present work was designed to investigate the preliminary phytochemical, antibacterial and GCMS analysis of various parts of the plant. The medicinally compounds from crude extracts of leaf, stem and root portions were fractionated in different solvents (aqueous, chloroform, petroleum ether and ethanol) subjected to preliminary phytochemical and antibacterial activities. The potential extracts were analysed through FTIR and GCMS. Phytochemical screening of leaves, stem and root extracts of Indigofera subulata revealed the presence of alkaloids, Quinones, reducing sugars, saponins, terpenoids and tannins. The ethanol extract of leaves and stem was found to exhibit activity against Pseudomonas aeruginosa Fourteen compounds were identified by GC-MS analysis. Phytomedicines avenues for the identifications of medicinally significant compounds with potential activity and it will lead to the isolation of potential antibiotics. Thus Indigofera subulata can be used as multi resistant drug in future.