Abstract: Effect of nitrogen (N) rate and its split application (AP) on qualitative and quantitative characteristics of sugarcane (Saccharum officinarum L.) cultivar CP48-103 was investigated on a loamy soil texture from 2006 to 2008 in the Sugarcane Research Center of the Khuzestan Province, Iran. The experiment was arranged in split plot randomized complete block design with three replications consisted of three different rates of N (N1 = 92, N2 = 138, N3 = 184 kg N ha-1) as main plots and three different AP (AP1 = 20-40-40%, AP2 = 30-35-35%, AP3 = 30-30-40%) as subplots. Twenty canes were randomly harvested from each plot and their quantitative and qualitative characteristics were determined. The results showed that both rate and split application of N fertilizer had no significant effect on sugarcane characteristics. The interactive effects of N application rate and AP on juice purity depicted applying 92 kg N ha-1 and AP of 30-30-40% gave the purest juice with 90%. The Nitrogen Use Efficiency (NUE) was significantly greater for N1 with values of 1.39 and 0.13 t kg-1 N in Cane Yield (CY) and Sugar Yield (SY), respectively. The results showed that the highest cane and sugar yield was obtained with 92 kg N ha-1 and AP of 30-35-35%.