Abstract: The research was carried out to investigate the cultivation of oyster mushroom (Pleurotus ostreatus) on different substrates. The steps involved in the cultivation were composting the substrates, bagging the substrates, sterilizing the bagged compost, spawning, incubation and cropping. The experiment was laid out in a completely randomized design with five treatments and six replications. Pinhead formation was faster (12 days), on the substrate made from grounded corn cob and the fruiting bodies formed in 27 days. The average number of fruiting bodies on the corn cob substrate was 28. Similarly, the fresh weight and dry weight of the fruiting bodies were all high for corncob substrate. These were in line with the figures of the biological efficiency which was higher for the corn cob substrate.