
	Trends in Applied Sciences Research
	Year: 2011 \| Volume: 6 \| Issue: 8 \| Page No.: 863-876

Development of Delivery Cargoes for Debriding Enzymes Effective in Wound Healing

Deependra Singh, Manju Rawat Singh, Swarnlata Saraf and S. Saraf

Abstract: The aim of present study was to apply experimental design methodology in the development and optimization of carrier system for Serratiopeptidase by modified double emulsion solvent evaporation technique. A three-level three-factorial Box-Behnken experimental design was used to characterize and optimize three physicochemical parameters-Eudragit RS100 concentration, external aqueous phase volume and stirring speed of primary emulsion on the entrapment and size of microspheres. The Response Surface Methodology (RSM) and multiple response optimization utilizing the polynomial equation were used to select optimal formulation with maximum entrapment and particle size in range. The maximum entrapment (80.62±1.96%) was achieved with 300 mg Eudragit RS100, 100 mL EAP and 2000 rpm as stirring speed and the observed responses coincided well with the predicted values from the RSM optimization technique. In vitro proteolytic activity confirmed the bioactivity of peptide after microencapsulation. The drug release from formulations showed a similar sustained release showing an initial burst followed by diffusion. In conclusion, a novel, controlled-release delivery system for peptide drug was successfully developed by experimental design methodology with the fewest number of experiments.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Recent Nanoparticulate Approaches of Drug Delivery for Skin Cancer

•	Evaluation of Ceftriaxone Releasing from Microspheres Based on Starch Against Salmonella spp.

•	Efficacy of Papain-based Wound Cleanser in Promoting Wound Regeneration

•	Poly [D, L-lactide-co-glycolide] Microspheres as a Delivery System of Protein Ovalbumin Used as a Model Protein Drug

How to cite this article:

Deependra Singh, Manju Rawat Singh, Swarnlata Saraf and S. Saraf, 2011. Development of Delivery Cargoes for Debriding Enzymes Effective in Wound Healing. Trends in Applied Sciences Research, 6: 863-876.

URL: https://scialert.net/abstract/?doi=tasr.2011.863.876

COMMENT ON THIS PAPER

Navigation


		Previous Issues
		Submit a Manuscript

Science Alert