Solubilization of Tricalcium Phosphate by Fungus Aspergillus niger at Different Carbon Source and Salinity

Research Article

**Solubilization of Tricalcium Phosphate by Fungus Aspergillus niger at Different Carbon Source and Salinity**

Janardan Yadav, Jay Prakash Verma and Kavindra Nath Tiwari

Plant Growth Promoting Fungus (PGPF) isolated from rhizosphere of chickpea and identified as Aspergillus niger strain BHUAS01 was tested for its tricalcium phosphate solubilizing ability at different sources of carbon viz., glucose, sucrose, glycerol and mannitol in Pikovskaya broth. Also, A. niger was analyzed solubilization of tricalcium phosphate (TCP) in broth media at different salinity viz., 1% NaCl, 1% KCl and 1% CaCl₂ at varying range of reaction (pH) under in vitro condition. Among the carbon sources, Aspergillus niger was found to solubilize maximum tricalcium phosphate (512 μg mL^-1) at glucose as carbon source and minimum activity (348 μg mL^-1) of phosphate solubilization at sucrose as carbon. Aspergillus niger showed maximum significant solubilization of tricalcium phosphate in Pikovaskaya broth containing carbon source glucose followed by glycerol, maltose and sucrose at 21 days of incubation. Further the effect of different salinity (1% NaCl, 1% KCl and 1% CaCl₂) was tested at different pH (6.0, 7.0 and 8.0) under in vitro condition. A. niger strain BHUAS01 was showed maximum significant solubilization of tricalcium phosphate (495 μg mL^-1) in presence of 1% CaCl₂ in modified Pikovskaya broth at pH 8.0 than other salt concentration. This finding can provide great benefit in the maintaining the available phosphates for crops in saline and alkaline soils. A large fraction of land arid and semiarid regions is affected by salinity in India.

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Janardan Yadav, Jay Prakash Verma and Kavindra Nath Tiwari, 2011. Solubilization of Tricalcium Phosphate by Fungus Aspergillus niger at Different Carbon Source and Salinity. Trends in Applied Sciences Research, 6: 606-613.

URL: https://scialert.net/abstract/?doi=tasr.2011.606.613

Comments

Sanjay Joshi Reply

13 October, 2012

I tried it.How it is possible,enzyme activity ends at 10 days only after wards it shows decrease in OD.
Please clarify it.

JAY PRAKASH VERMA

19 October, 2012

Sanjay ji, In our publication, enzyme activity will depend on the microbial physiology and their growth activities. In this publication, "solubilization of Tricalcium Phosphate by Fungus Aspergillus niger at Different Carbon Source and Salinity" I have study the solubilization capability, with different ph variation and carbon sources at 7, 14 and 21 days incubation. In this paper, Aspergillus grow continuously in the medium till the carbon source is available for growth.I am not determinate the enzyme activities, but I am sure, the enzyme activities enhance at time of fungal growth and large biomass production. when the nutrient is decreases then the fungal growth will also decrease, by which the enezyme activities also decreases. Enzyme activities will also depend on the carbon source and specific ph of the medium for growth of the microbial cell.

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Authors

Janardan Yadav

Jay Prakash Verma

Kavindra Nath Tiwari

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