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Pharmacognostical Evaluation of Ethanolic, Petroleum Ether and Distilled Water Extract of Woodfordia fruticosa, Cissampelos pareira and Stephania glabra |
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Hem Raj,
Avneet Gupta and Neeraj Upmanyu |
Abstract:
The idea for the present study is taken up from the folklore uses of Woodfordia fruticosa (WFE), Cissampelos pareira (CPE) and Stephania glabra (SGE). These plants are used to treat different ailments in tribal areas of Nepal, Himalayan region of India and China. Knowing their historical medicinal value, present study is focused on pharmacognostical study of different extracts from leaves of WFE, CPE and rhizome of SGE. Powder of dried leaves of WFE and CPE and rhizome of SGE were extracted by soxhlet apparatus using petroleum ether and ethanol as solvent. These plants were also extracted with distilled water by maceration. Thin Layer Chromatography (TLC) was performed for distilled water extracts, Hydrodistillation of 30 g dried leaves powder of CPE were performed by using clevenger apparatus. The phytochemical screening showed the presence of flavonoids, carbohydrate in all the extracts. The amount of volatile oil was reported to be 0.33%. Rf values in TLC plates from solvent system Toluene: Ethyl acetate was reported as 0.18, 0.077, 0.155 for SGE, 0.166, 0.24 for WFE and 0.5 and 0.488 for CPE. Rf values from solvent system Hexane: Chloroform: Methanol were reported as 0.126, 0.294, 0.642, 0.631 and 0.894 from CPE. In this solvent system Rf values for WFE were reported as 0.085, 0.0571, 0.042 and for SGE as 0.075, 0.1, 0.125, 0.15, 0.187 and 0.212. From the above study it was concluded that these studies could be useful for identification and preparation of a monograph of these plants.
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How to cite this article:
Hem Raj, Avneet Gupta and Neeraj Upmanyu, 2014. Pharmacognostical Evaluation of Ethanolic, Petroleum Ether and Distilled Water Extract of Woodfordia fruticosa, Cissampelos pareira and Stephania glabra. Singapore Journal of Scientific Research, 4: 1-8.
DOI: 10.3923/sjsres.2014.1.8
URL: https://scialert.net/abstract/?doi=sjsres.2014.1.8
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