Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Science International
  Year: 2014 | Volume: 2 | Issue: 3 | Page No.: 72-75
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

PR-Proteins Accumulation in Tomato Plant Due to Application of Resistance Inducing Chemicals During Period of Induced Resistance Against Alternaria Leaf Blight

S.A. Raut and S.G. Borkar

Abstract:
Background: Resistance Inducing Chemicals (RIC) viz. Salicylic Acid (SA), β-aminobutyric acid (BABA), chitosan (CHT) and 2,6-dichloroisonicotinic acid (INA) are known to play an important role in induction of resistance by increasing the activity of PR-proteins. Therefore, the study was done to test these RIC’s for accumulation of PR-proteins in tomato plant to confer resistance against Alternaria blight disease. Methods: The activity of PR-proteins viz. chitinase and β 1,3-glucanase in tomato leaves were measured in response to treatment of these RIC’s at different concentrations as seed dip treatment for 8 h, seedling dip treatment for 2 h and seed+seedling treatment. The RIC treated plants were challenged inoculated with Alternaria alternata, a tomato leaf blight pathogen at 10 days interval from germination and observed for disease development. Chitinases and β 1,3-glucanase activity was determined spectronically by quantifying the release of mg of N-acetylglucosamine and of glucose respectively at 30 days of RIC treatment as the persistence of resistance induced by RIC was up to 50 days. Results: Maximum activity of chitinase and β 1,3-glucanase was observed in seed+seedling treatment with BABA @ 15 mM concentration and the increase in chitinase and β 1,3-glucanase activity was 32.00 and 56.59% more respectively over control and was followed by treatment with salicylic acid @ 1.5 mM concentration. Conclusion: The application of RIC SA and BABA elicits the increased activity of PR-proteins viz. chitinase and β 1,3-glucanase which inhibit the process of pathogenesis in susceptible tomato cultivars to exhibit the resistance.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Exogenous Applications of Salicylic Acid for Inducing Systemic Acquired Resistance Against Tomato Stem Canker Disease
How to cite this article:

S.A. Raut and S.G. Borkar, 2014. PR-Proteins Accumulation in Tomato Plant Due to Application of Resistance Inducing Chemicals During Period of Induced Resistance Against Alternaria Leaf Blight. Science International, 2: 72-75.

URL: https://scialert.net/abstract/?doi=sciintl.2014.72.75

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
Curve Bottom