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Research Journal of Veterinary Sciences
  Year: 2012 | Volume: 5 | Issue: 3 | Page No.: 75-80
DOI: 10.3923/rjvs.2012.75.80
 
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Validation of Commercial Test Kit for Microbiological Screening of Antimicrobials in Chicken Eggs

I.O. Fagbamila, S.S. Ngulukun, S.S. Ardzard, N. Sati, O.T. Ajayi, P.I. Ankeli, Y. Akalusi, L. Okeke, L. Ikpa, M.O. Odugbo and M. Muhammad

Abstract:
The present study was conducted to evaluate a commercial test kit for the qualitative screening of eggs submitted to our laboratory as a first step for the testing of drug residues. Forty hens at the point of lay were kept for 4 weeks without administering any antibiotic. Eggs were then collected and tested for the absence of drug residues. Antibiotic-free birds were then divided into two groups. One group was administered tetracycline for three days and the other group left as control. Eggs were collected daily for two weeks from both groups and tested for tetracycline residues using the disc diffusion method and a commercial test kit. Both methods detected the presence of drug residues in test eggs with the commercial test kit able to detect residues over a longer period up to day 10. The study indicated that the commercial test kit could be used for the detection of drug residues particularly when the aim is to screen large numbers of samples rapidly. However, it is not sensitive enough to detect drug residues at lower concentrations and may not be suitable for confirmatory testing.
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How to cite this article:

I.O. Fagbamila, S.S. Ngulukun, S.S. Ardzard, N. Sati, O.T. Ajayi, P.I. Ankeli, Y. Akalusi, L. Okeke, L. Ikpa, M.O. Odugbo and M. Muhammad, 2012. Validation of Commercial Test Kit for Microbiological Screening of Antimicrobials in Chicken Eggs. Research Journal of Veterinary Sciences, 5: 75-80.

DOI: 10.3923/rjvs.2012.75.80

URL: https://scialert.net/abstract/?doi=rjvs.2012.75.80

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