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Research Journal of Phytochemistry
  Year: 2017 | Volume: 11 | Issue: 1 | Page No.: 20-27
DOI: 10.3923/rjphyto.2017.20.27
 
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A Comparative Evaluation of Microwave and Conventional Soxhlet Extraction Methods for the Antioxidant, Hypoglycemic and Hypolipidemic Potentials of Jordanian Psidium guajava Raw Fruit Peel Extracts

R. Issa , S. Khater and M. Abu- Samak

Abstract:
Background: Psidium guajava L. (Myrtaceae) is one of the medicinal plants in Jordan that have been reported in the folk medicine for different indications and few of them have been experimentally and/or clinically determined. Objective: The current study aims to explores the aqueous extracts obtained from Jordanian premature P. guajava fruit peel (PGFP), using two different methods for extraction. Also to assess these extracts different pharmacological activities as well as their phytochemistry. Materials and Methods: Two different methods for extraction were used, Microwave-Integrated Soxhlet (MIS) and Conventional Soxhlet (CS) extraction methods. The TLC analysis was used to optimize each extraction method and for the preliminary phytochemical screening. The HPLC-MS/MS analysis allowed the identification and quantification of the phenol compounds in the PGFP two extracts. For each extract type, the antioxidant activity was evaluated using the ABTS method. In addition, the hypoglycemic and hypolipidemic potentials for each extract were also evaluated on normoglycemic and streptozotocin-treated diabetic rats. Statistical analysis was performed using the one-way analysis of variance (ANOVA) in order to reveal significant (p<0.05) differences between the tested animal groups. Results: The CS extract showed ellagic acid as the major constituent, followed by gallic, P-coumaric, furelic acids, quercetin and ascorbic acid in order of their percentage content (total phenol content). Regarding the MIS extract, ascorbic acid was detected as the major compound followed by gallic, P-coumaric acids and quercetin. Moreover, MIS extract showed to contain higher level of total phenol compounds. Comparison of pharmacological studies showed more potent effects of CS as antioxidant and hypolipidemic extract, where the MIS extract showed higher hypoglycemic effect. Conclusion: The presented data suggest that PGFP can be used for their antioxidant, hypolipidemic and hypoglycemic effects, if the extraction methods were optimized for the required pharmacological effect. In this study, it was correlated that, significant hypolipidemic effect of unripe guava to high content of ellagic acid in its fruit peels and antioxidant action of the active extract. This effect for ellagic acid seems to be mediated by metabolic pathway by which it acts as antilypolytic agent to reduce the consequences of diabetes mellitus, such as atherosclerosis rather than antidiabetic effect.
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How to cite this article:

R. Issa, S. Khater and M. Abu- Samak, 2017. A Comparative Evaluation of Microwave and Conventional Soxhlet Extraction Methods for the Antioxidant, Hypoglycemic and Hypolipidemic Potentials of Jordanian Psidium guajava Raw Fruit Peel Extracts. Research Journal of Phytochemistry, 11: 20-27.

DOI: 10.3923/rjphyto.2017.20.27

URL: https://scialert.net/abstract/?doi=rjphyto.2017.20.27

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