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Research Journal of Phytochemistry

Year: 2012 | Volume: 6 | Issue: 1 | Page No.: 9-16
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Authors

F. Faparusi

M.M. Bello-Akinosho

R.T. Oyede

A. Adewole

P.O. Bankole

F.F. Ali

Keywords

Brillantaisia patula is a medicinal plant used for different ailments in Africa. Phytochemical constituents and antibacterial potential of the plant were investigated. In vitro antibacterial activity using agar-well diffusion method was carried out against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 24212), Proteus hauseri (ATCC 13315), Pseudomonas aeruginosa ATCC 27853 and Escherichia coli (ATCC 38218). The phytochemistry of both methanol and ethanol extracts of Brillantaisia patula leaf revealed the presence of alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins, steroids, flavonoids and saponins. Terpenoids and flavonoids were found in the methanolic extract but not in the ethanolic extract while steroid was found in the ethanolic extract but not in the methanolic extract. The ethanolic extract was active against all the five pathogenic bacteria while the methanolic extract inhibited all the test bacteria but Staphylococcus aureus. Ethanolic extract zones of inhibition ranged from 15.3±0.6 to 20.7±0.6 mm, whereas methanolic extract zones of inhibition ranged from 18.0±1.0 to 30.0±1.0 mm. The Minimum Inhibitory Concentration (MIC) of ethanolic extract ranged from 25 to 200 mg mL-1 while that of methanolic extract was from 50 to 100 mg mL-1. The Minimum Bactericidal Concentration (MBC) of methanolic extract ranged from 100 to 200 mg mL-1. The least MBC value (50 mg mL-1) of ethanolic extract was against Escherichia coli while the highest value (>200 mg mL-1) was against Proteus hauseri. Leaf of Brillantaisia patula could be a novel source of antibacterial agent (s) that might have broad spectrum activity.
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How to cite this article

F. Faparusi, M.M. Bello-Akinosho, R.T. Oyede, A. Adewole, P.O. Bankole and F.F. Ali, 2012. Phytochemical Screening and Antibacterial Activity of Brillantaisia patula Leaf. Research Journal of Phytochemistry, 6: 9-16.

URL: https://scialert.net/abstract/?doi=rjphyto.2012.9.16

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