Science Alert
Curve Top
Research Journal of Immunology
  Year: 2011 | Volume: 4 | Issue: 1 | Page No.: 31-37
DOI: 10.3923/rji.2011.31.37
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Probiotic Action of Lactobacillus Isolated from the Milk Sample against Some Human Pathogens

M. Premalatha and P. Dhasarathan

Probiotic efficiency of lactobacillus strains was isolated from milk sample and their antibacterial activities against human bacterial pathogens were screened in the present study. The milk samples were collected in sterile containers from different places of Tamil Nadu, India. The samples were analysed qualitatively and microbiologically. Lactobacillus was isolated from the milk samples and it was identified by biochemical tests. Methylene blue reduction test, which is colour sensitive to oxygen concentration is added to the milk. Resazurin test used to screen milk quality based either on the colour production. The total bacterial population count of milk sample was enumerated by Pour plate technique. MRS agar plates were prepared and a loopful of milk sample is taken. Quadrant streak procedure was carried out to isolate Lactobacillus. The isolated strains are identified and used to study their antagonistic efficiency against human bacterial pathogens isolated from different pathologic medium from patients diagnosed to have various wound infection at the laboratory Joys, Nagercoil, Kanyakumari (district) Tamilnadu. The probiotic action of Lactobacillus was studied against some human pathogens like Eschericia coli, Staphylococcus aureus, Enterobacter aerogenes, Streptococcus pyogenes, Pseudomonas aeruginosa and Salmonella typhimurium and it was observed that Lactobacillus showed good antagonistic effect against these pathogens.
PDF Fulltext XML References Citation Report Citation
  •    Isolation and Phenotypic Characterization of Lactobacillus Species from Various Dairy Products
  •    Immunomodulatory Effects of Multistrain Probiotics (Protexin™) on Broiler Chicken Vaccinated Against Avian Influenza Virus (H9)
  •    Inhibition of Growth of Escherichia coli, Salmonella typhimurium, and Clostridia perfringens on Chicken Feed Media by Lactobacillus salivarius and Lactobacillus plantarum
  •    Antagonistic Effects on Enteropathogens and Plasmid Analysis of Lactobacilli Isolated from Fermented Dairy Products
  •    Therapeutic Properties of Probiotic Bacteria
How to cite this article:

M. Premalatha and P. Dhasarathan, 2011. Probiotic Action of Lactobacillus Isolated from the Milk Sample against Some Human Pathogens. Research Journal of Immunology, 4: 31-37.

DOI: 10.3923/rji.2011.31.37






Curve Bottom