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Research Journal of Environmental Sciences
  Year: 2014 | Volume: 8 | Issue: 3 | Page No.: 123-133
DOI: 10.3923/rjes.2014.123.133
 
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Isolation and Identification of Klebsiella pneumonia and Klebsiella oxytoca Bacteriophages and their Application in Wastewater Treatment and Coliform’s Phage Therapy

Keivan Beheshti Maal, Abbas Soleimani Delfan and Sharareh Salmanizadeh

Abstract:
Recently prevention of bacterial infections using their specific viruses, bacteriophages, is challenging. In this research we isolated and identified a native strain of Klebsiella pneumoniae from Isfahan municipal wastewater treatment plant we named K. pneumoniae SBSWIF3. Also we isolated and characterized two novel bacteriophages related to Myoviridae and Podoviridae families of bacteriophages from Zayandehrood River, Isfahan, Iran that had lytic effects on K. pneumoniae SBSWIF3, K. pneumonia PTCC1290 and K. oxytoca PTCC1402 as coliform's index. The Myovirus had a hexagonal head measuring 27.28 nm and a noncontractile tail measuring 204.5x13.63 nm. The Podovirus had an oval head measuring 98x35 nm and a tail with 14 nm in diameter. The treatment of municipal sewage with the coliphage mixture resulted in 22 folds decrease of the coliform’s MPN from 2400-110 after 2 h incubation. We suggested that the use of these lytic coliphages for reduction of coliform’s population in sewage could be considered as an effective and simple alternative for costly replacement of instruments and establishments of the old wastewater treatment plants.
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How to cite this article:

Keivan Beheshti Maal, Abbas Soleimani Delfan and Sharareh Salmanizadeh, 2014. Isolation and Identification of Klebsiella pneumonia and Klebsiella oxytoca Bacteriophages and their Application in Wastewater Treatment and Coliform’s Phage Therapy. Research Journal of Environmental Sciences, 8: 123-133.

DOI: 10.3923/rjes.2014.123.133

URL: https://scialert.net/abstract/?doi=rjes.2014.123.133

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