Submit Manuscript

Pakistan Journal of Nutrition

Year: 2005 | Volume: 4 | Issue: 4 | Page No.: 237-241
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 401

Search

Authors

Bilge Hilal Cadirci

Sumru Citak

Keywords

Research Article

A Comparison of Two Methods Used for Measuring Antagonistic Activity of Lactic Acid Bacteria

Bilge Hilal Cadirci and Sumru Citak
In this research, we have aimed to determine antagonistic effects of various lactic acid bacteria against Gram (+) and Gram (-) bacteria with a comparison of disc diffusion and spot-on-lawn method. In spot-on-lawn method, P. aeruginosa ATCC 27853 was the most sensitive of the tested bacteria, followed by E. coli ATCC 25927 and P. aeruginosa ATCC 10145. On the other hand, in disc diffusion assay B. subtilis ATCC 6633, E. coli O157:H7 and P. aeruginosa ATCC 10145 were the most sensitive indicator microorganisms. The spot-on-lawn method was suitable for assessing the antagonistic effect of lactic acid bacteria. In general, inoculum density had little effect on inhibition.
PDF Citation

Related Articles

Isolation, Purification and Characterization of Antimicrobial Agent Antagonistic to Escherichia coli ATCC 10536 Produced by Bacillus pumilus SAFR-032 Isolated from the Soil of Unaizah, Al Qassim Province of Saudi Arabia
Anti-Vibrio harveyi Property of Micrococcus luteus Isolated from Rearing Water under Biofloc Technology Culture System
Effect of Lactobacillus lactis cremoris Isolated from Kefir against Food Spoilage Bacteria
Study of Physical and Cultural Parameters on the Bacteriocins Produced by Lactic Acid Bacteria Isolated from Traditional Indian Fermented Foods

Leave a Comment

Your email address will not be published. Required fields are marked *