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Pakistan Journal of Biological Sciences
  Year: 2014 | Volume: 17 | Issue: 5 | Page No.: 735-739
DOI: 10.3923/pjbs.2014.735.739
 
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Production of Cellulase Enzyme by Trichoderma reesei Cef19 and its Application in the Production of Bio-ethanol

M. Ravi Kumar, M.D. Bala Kumaran, P. Balashanmugam, A. Immaculate Nancy Rebecca, D.J. Mukesh Kumar and P.T. Kalaichelvan

Abstract:
The present study aimed at the production of cellulase enzyme from the cellulolytic fungi Trichoderma reesei CEF19 and subsequent application of the cellulase for the fermentation of ethanol. For the same, the cellulolytic fungi, Trichoderma reesei CEF19 was isolated and was allowed to produce cellulase enzyme using optimized conditions. The cellulase enzyme was extracted and purified with the help of ammonium sulphate precipitation, dialysis followed by ion exchange chromatography with DEAE-Sephadex column. The purified cellulase enzyme was characterized using SDS-PAGE analysis. The saccharification of the cellulosic substrates was done using the cellulase enzyme. The fermentation of saccharified cellulosic substrates into ethanol was carried out using Saccharomyces cerevisiae. From the results obtained, rice straw was found to be the better source for the ethanol production when compared to the other substrates.
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How to cite this article:

M. Ravi Kumar, M.D. Bala Kumaran, P. Balashanmugam, A. Immaculate Nancy Rebecca, D.J. Mukesh Kumar and P.T. Kalaichelvan, 2014. Production of Cellulase Enzyme by Trichoderma reesei Cef19 and its Application in the Production of Bio-ethanol. Pakistan Journal of Biological Sciences, 17: 735-739.

DOI: 10.3923/pjbs.2014.735.739

URL: https://scialert.net/abstract/?doi=pjbs.2014.735.739

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