Production and Optimization of Indole Acetic Acid by Indigenous Micro Flora using Agro Waste as Substrate

Research Article

Production and Optimization of Indole Acetic Acid by Indigenous Micro Flora using Agro Waste as Substrate

M. Sudha, R. Shyamala Gowri, P. Prabhavathi, P. Astapriya, S. Yamuna Devi and A. Saranya

Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and identified as Rhizobium sp. and Bacillus sp., Optimization of Indole acetic acid production was carried out at different cultural conditions, such as pH, temperature and substrate with Rhizobium sp., Bacillus sp. and Rhizobium sp., produced higher amount of Indole acetic acid (6.1 mg mL^-1) than the Bacillus sp., (4.4 mg mL^-1) at pH 7 and 37°C in the Bengal gram substrate. Partial purification of Indole acetic acid was done by Thin Layer Chromatography (TLC). In conclusion Rhizobium sp., appear to be a suitable soil microorganism for high level of IAA production.

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M. Sudha, R. Shyamala Gowri, P. Prabhavathi, P. Astapriya, S. Yamuna Devi and A. Saranya, 2012. Production and Optimization of Indole Acetic Acid by Indigenous Micro Flora using Agro Waste as Substrate. Pakistan Journal of Biological Sciences, 15: 39-43.

DOI: 10.3923/pjbs.2012.39.43

URL: https://scialert.net/abstract/?doi=pjbs.2012.39.43

Pakistan Journal of Biological Sciences

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Authors

M. Sudha

R. Shyamala Gowri

P. Prabhavathi

P. Astapriya

S. Yamuna Devi

A. Saranya

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Research Article

Production and Optimization of Indole Acetic Acid by Indigenous Micro Flora using Agro Waste as Substrate

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