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Pakistan Journal of Biological Sciences

Year: 2011 | Volume: 14 | Issue: 10 | Page No.: 578-583
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Authors

J. Sakande

P. Rouet-benzineb

H. Devaud

J.B. Nikiema

M. Lompo

O.G. Nacoulma

I.P. Guissou

A. Bado

Keywords

Borassus aethiopum MART (Arecaceae) is a plant used in traditional herbal medicine for the treatment of various diseases (bronchitis, laryngitis, antiseptic). In particular, their male inflorescences were reported to exhibit cicatrizing, antiseptic and fungicidal properties. In the present study, the biological activity of E2F2, an apolar extract from Borassus aethiopum male inflorescence was investigated on colon cancer HT29 cells. Phytochemical screening was carried according to methodology for chemical analysis for vegetable drugs. Cells proliferation was determined by the MTT assay and cells cycle distribution was analysed by using laser flow cytometer (Beckman coulter). The cytoskeleton organisation was examined under a laser scanning confocal microscope (Zess). Preliminary phytochemical analysis of E2F2 extract revealed the presence of sterols, triterpenes and saponosids. E2F2 extract (1 μg and 100 μg mL-1) significantly inhibited cell proliferation by blocking cell population in G0/G1 phase. Flow Cytometric analysis of E2F2-treated HT29 cells showed that hypoploïd cell population (sub G1 phase) increased with processing time exposures. Immunofluorescence confocal analysis revealed a disrupt actin microfilaments network in E2F2 treated-cells with a significant reduction in actin stress fibres and appearance of a random, non-oriented distribution of focal adhesion sites. These data indicate that E2F2 extract has anti-proliferative and pro-apoptotic activities. Further studies are required to unravel the mechanisms of action of E2F2 extract.
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How to cite this article

J. Sakande, P. Rouet-benzineb, H. Devaud, J.B. Nikiema, M. Lompo, O.G. Nacoulma, I.P. Guissou and A. Bado, 2011. Dichloromethane-methanol Extract from Borassus aethiopum Mart. (Arecaceae) Induces Apoptosis of Human Colon Cancer Ht-29 Cells. Pakistan Journal of Biological Sciences, 14: 578-583.

DOI: 10.3923/pjbs.2011.578.583

URL: https://scialert.net/abstract/?doi=pjbs.2011.578.583

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