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Pakistan Journal of Biological Sciences
  Year: 2009 | Volume: 12 | Issue: 6 | Page No.: 530-533
DOI: 10.3923/pjbs.2009.530.533
 
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Evaluation of Excreted/Secreted Antigens Derived from Peritoneal of Toxoplasma Infected Small Mice to Detect IgG Against Toxoplasma

Seyed Hossein Abdollahi, Mohammad Kazemi Arababadi and Gholamhossein Hassanshahi

Abstract:
The present study, evaluated, using the components of peritoneal fluid of infected mice (as another source of E/SA), for the detection of Toxoplasma specific IgG in human serum. Peritoneal fluids of mice infected by intraperitoneal (IP) inoculation of Toxoplasma tachizoites were collected after 3 days and centrifuged at 750 x g for 15 min then the supernatant was precipitated with ammonium sulphate solution (40% saturated) and used as components containing E/SA. Forty nine uninfected ( without anti-Toxoplasma antibodies) and thirty two positive (with IgG to Toxoplasma) human serum samples were selected (all sera were first tested by standard method for detection of IgG antibodies anti-T. gondii) then the sera were tested by ELISA using E/SA. The cut-off point with 95% confidences was found to be 0.78. Moreover, sensitivity and specificity of the method were determined to be 84 and 92%, respectively. The present results indicate that peritoneal exudates from mice infected with T. gondii may be used as a source of antigenic material for the detection of Toxoplasma-specific IgG and may be valuable for the development of new tools in the serodiagnosis of toxoplasmosis.
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How to cite this article:

Seyed Hossein Abdollahi, Mohammad Kazemi Arababadi and Gholamhossein Hassanshahi, 2009. Evaluation of Excreted/Secreted Antigens Derived from Peritoneal of Toxoplasma Infected Small Mice to Detect IgG Against Toxoplasma. Pakistan Journal of Biological Sciences, 12: 530-533.

DOI: 10.3923/pjbs.2009.530.533

URL: https://scialert.net/abstract/?doi=pjbs.2009.530.533

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