Isolation and Identification of a Lipase Producing Bacillus sp. from Soil

Research Article

**Isolation and Identification of a Lipase Producing Bacillus sp. from Soil**

Kambiz Morabbi Heravi, Fereshteh Eftekhar, Bagher Yakhchali and Fatemeh Tabandeh

Lipase production in an indigenous lipolytic Bacillus sp. was detected in media containing Tributyrin-Tween 80 and Rhodamine B-Olive oil. The statistical Taguchi model was used to predict the optimum experimental conditions for bacterial growth and lipase production. Partial optimization was carried out for selection of salt base, oil, glucose, NH₄Cl and yeast extract concentrations, inoculum density, pH and agitation. Maximum lipase activity was detected in the cell free supernatants of cultures grown in a medium containing 10 g L^-1 yeast extract, 15 g L^-1 NH₄Cl, 3 g L^-1 K₂HPO₄, 1 g L^-1 KH₂PO₄, 0.1 g L^-1 MgSO₄.7H₂O, 2 g L^-1 glucose, 0.6 mM MgCl₂ and 15 ml L^-1 olive oil, pH 8.5 at 30 °C for 24 h and low agitation. The amount of lipase produced in the designed medium was in agreement with the predicted values by the statistical method. 16S rRNA cloning and sequencing identified the test organism as Bacillus pumilus.

PDF Fulltext XML References Citation

How to cite this article

Kambiz Morabbi Heravi, Fereshteh Eftekhar, Bagher Yakhchali and Fatemeh Tabandeh, 2008. Isolation and Identification of a Lipase Producing Bacillus sp. from Soil. Pakistan Journal of Biological Sciences, 11: 740-745.

DOI: 10.3923/pjbs.2008.740.745

URL: https://scialert.net/abstract/?doi=pjbs.2008.740.745

Pakistan Journal of Biological Sciences

Article Trend

Total views 442

Authors

Kambiz Morabbi Heravi

Fereshteh Eftekhar

Bagher Yakhchali

Fatemeh Tabandeh

Keywords

Research Article

**Isolation and Identification of a Lipase Producing Bacillus sp. from Soil**

How to cite this article

Related Articles