
	Pakistan Journal of Biological Sciences
	Year: 2007 \| Volume: 10 \| Issue: 5 \| Page No.: 768-772 DOI: 10.3923/pjbs.2007.768.772

Effects Modification of Iron Hematoxylin on Neuron Staining

A.M. Gharravi, M.J. Golalipour, R. Ghorbani and M. Khazaei

Abstract: Iron weigert staining methods is used as nuclear staining. In present study we introduce a modification iron weigert hematoxylin for staining neuron without astrocytes. Whole brain of adult wistar rats (12-1 3 week old) were removed, immersed in formaldehyde fixative and embedded in paraffin. Sections, 5-7 μm (from brain cortex, hippocampus, cerebellum) divided to three groups: one for staining by Hematoxyllin and eosin, second for staining by cresyl fast vio let (that specially performed for Nisl substances in neuron) and last for staining by modification iron hematoxyllin methods, but different in quantity and quality. In new method general and specific architecture of neuron, nucleus and nuclear envelope was clearly visible reactions of neuron were predominant. Astrocyte did not respond to staining methods. Also spines (axon) of purkinje cells clearly visible. Modification iron weigert hematoxylin can be replacement to cost and time consuming chemical staining method for staining neurons

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Some Histological Observations and Microstructural Changes in the Nissl Substances in the Cerebellar Cortex of Adult Wistar Rats following Artesunate Administration

How to cite this article:

A.M. Gharravi, M.J. Golalipour, R. Ghorbani and M. Khazaei, 2007. Effects Modification of Iron Hematoxylin on Neuron Staining. Pakistan Journal of Biological Sciences, 10: 768-772.

DOI: 10.3923/pjbs.2007.768.772

URL: https://scialert.net/abstract/?doi=pjbs.2007.768.772

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Science Alert