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Pakistan Journal of Biological Sciences

Year: 2007 | Volume: 10 | Issue: 11 | Page No.: 1918-1921
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N. Trachoo

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Research Article

Attachment of Listeria monocytogenes, Escherichia coli O157:H7 and Pseudomonas aeruginosa on Food Soiled Plastic Surfaces

N. Trachoo
This study investigated the ability of three different pathogens, Escherichia coli O157:H7, Listeria monocytogenes and Pseudomonas aeruginosa, to attach food soiled (salad dressing, cooking oil, milk and yogurt) polyvinyl chloride (PVC) plastic. The pre-soiled PVC coupons were incubated with 108 CFU/cm2 of each bacterium in 50% tryptic soy broth for 6 h to allow attachment. It was found that the effect of food products was not significant while the effect of bacterial strain was highly significant (p = 0.0005). Mean attachment of P. aeruginosa (5.5 log CFU/cm2) to pre-soiled coupons was the highest (p<0.05), compared to L. monocytogenes (5.0 log CFU/cm2) and E. coli O157:H7 (4.5 log CFU/cm2). This study indicates the capability of each bacterial pathogen, E. coli O157:H7, L. monocytogenes or P. aeruginosa, to equally attach to different pre-soiled PVC surfaces. P. aeruginosa showed the greatest potential to attach to PVC when compared to E. coli O157:H7 and L. monocytogenes.
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