
	Pakistan Journal of Biological Sciences
	Year: 2006 \| Volume: 9 \| Issue: 2 \| Page No.: 302-306 DOI: 10.3923/pjbs.2006.302.306

In vitro Plant Regeneration from Callus of Cotyledons in Canola (Brassica napus L.)

F. Chamandosti, A. Majd and S. Mehrabian

Abstract: In this study a simple and efficient procedure was developed for inducing callus and plant regeneration using hypocotyl segmsents of Canola (Brassica napus L.). The combinations and concentrations of differents plant growth regulators had a critical effect for callus formation with various colour and potential of differentiation. Three morphologically distinct types of calli were induced: 1) yellow calli were induced on MS medium supplemented with variou concentrations of 2, 4-D and 2 mg L^-1 BA and presence of somatic embryos in first stages of development was showed by histological studies in these calli. white calli were induced on MS medium supplemented with into 1-2 mg L^-1 IBA and 1 mg L^-1 BA and MS medium with only 2 mg L^-1 BA. These calli were organogen. So that differentiated to shoots, then shoots rooted and whole plants were obtained. And dark brown calli that induced on media with out of 2 mg L^-1 IBA. These calli died and did not differentiated.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	In vitro Plant Regeneration from Callus of Hypocotyls and Cotyledonary Explants in Ethiopian Mustard (Brassica carinata A. Braun), Yellow Dodolla Cultivar

•	In vitro Selection: A Novel Source of Resistance to Sclerotinia Stem Rot in Canola (Brassica napus L.)

How to cite this article:

F. Chamandosti, A. Majd and S. Mehrabian , 2006. In vitro Plant Regeneration from Callus of Cotyledons in Canola (Brassica napus L.). Pakistan Journal of Biological Sciences, 9: 302-306.

DOI: 10.3923/pjbs.2006.302.306

URL: https://scialert.net/abstract/?doi=pjbs.2006.302.306

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Science Alert