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Pakistan Journal of Biological Sciences

Year: 2004 | Volume: 7 | Issue: 2 | Page No.: 277-278
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Authors

Ihsan Ullah

Hamid Rashid

M. Ramzan Khan

Keywords

Research Article

Establishment of Tissue Culture Protocol in Brassica (B. napus L.)

Ihsan Ullah, Hamid Rashid and M. Ramzan Khan
Hypocotyl segments of rapeseed cultivar "Rainbow" were cultured on B5 medium supplemented with several concentrations of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) ranging from 0.0-4.0 mg l-1. Response to callus induction differed according to the levels of hormonal treatments. 2,4-D @1.5 mg l- induced growth of compact, green callus whereas other levels of 2,4-D produced poorly developed calli. Embryogenic calli were transferred to the medium supplemented with different combinations of Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) at levels of 0.0/0.0, 0.1/0.5, 1.0/0.5, 2.5/1.0, 5.0/1.0 and 5.0/2.0 mg l-1, respectively. More than 50% of the calli cultured on the medium supplemented with BAP@ 5.0 mg l-1 with NAA 1.0 mg l-1 regenerated into plantlets.
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