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Pakistan Journal of Biological Sciences
  Year: 2004 | Volume: 7 | Issue: 2 | Page No.: 277-278
DOI: 10.3923/pjbs.2004.277.278
 
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Establishment of Tissue Culture Protocol in Brassica (B. napus L.)

Ihsan Ullah, Hamid Rashid and M. Ramzan Khan

Abstract:
Hypocotyl segments of rapeseed cultivar "Rainbow" were cultured on B5 medium supplemented with several concentrations of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) ranging from 0.0-4.0 mg l-1. Response to callus induction differed according to the levels of hormonal treatments. 2,4-D @1.5 mg l- induced growth of compact, green callus whereas other levels of 2,4-D produced poorly developed calli. Embryogenic calli were transferred to the medium supplemented with different combinations of Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) at levels of 0.0/0.0, 0.1/0.5, 1.0/0.5, 2.5/1.0, 5.0/1.0 and 5.0/2.0 mg l-1, respectively. More than 50% of the calli cultured on the medium supplemented with [email protected] 5.0 mg l-1 with NAA 1.0 mg l-1 regenerated into plantlets.
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How to cite this article:

Ihsan Ullah, Hamid Rashid and M. Ramzan Khan , 2004. Establishment of Tissue Culture Protocol in Brassica (B. napus L.). Pakistan Journal of Biological Sciences, 7: 277-278.

DOI: 10.3923/pjbs.2004.277.278

URL: https://scialert.net/abstract/?doi=pjbs.2004.277.278

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