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Pakistan Journal of Biological Sciences

Year: 2002 | Volume: 5 | Issue: 6 | Page No.: 693-695
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Authors

Muhammad Ramzan Khan

Hamid Rashid

Azra Quraishi

Keywords

Research Article

Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar

Muhammad Ramzan Khan, Hamid Rashid and Azra Quraishi
Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B5) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential.
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