• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Pakistan Journal of Biological Sciences
  2. Vol 2 (4), 1999
  3. 1472-1477
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Pakistan Journal of Biological Sciences

Year: 1999 | Volume: 2 | Issue: 4 | Page No.: 1472-1477
DOI: 10.3923/pjbs.1999.1472.1477

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 199

Search


Authors


Rizwana Anwar


Shahid Karim


S. Riazuddin


Keywords


  • Bacillus thuringiensis
  • Cnaphalocrocis medinalis
  • Rice
  • PCR
  • toxicity
Research Article

Entomocidal Activity of Bacillus Thuringiensis Transgenic Rice Plants Against Rice Leaffolder, Cnaphalocrocis Medinalis (Lepidoptera: Pyralidae).

Rizwana Anwar, Shahid Karim and S. Riazuddin
Transgenic rice plants obtained through the incorporation of Bt cry1Ac gene. Initially putative transformants were screened for pesticidal activity against rice leaf folder larvae. Selected putative transformants showed a range of larval mortality from 20-100 percent to C. medinalis. The integration of the Bt cry gene was checked by Polymerase Chain Reaction (PCR), using primers 5` ACA GAA GAC CCT TCA ATA TC 3` and 3` GTT ACC GAG TGA AGA TGT AA 5` for amplification of a 656 bp fragment of DNA from the genomic DNA of putative transformants. Selection based on PCR and initial bioassays, two transgenic plants designated as, CAMB 1148 and CAMB 1150 were further confirmed for toxin expression by ELISA, Western blot analysis and comparative biotoxicity assay. ELISA and Western blot were performed with polyclonal antibody of Cry1Ac toxins that showed the presence of active ingredient of Bt toxin in both plants. Biotoxicity assays also reconfirmed the stability and activity of Cry toxins in both transgenic rice plants, eventually aiming for homozygosity with enhanced resistance to rice leaf folder.
PDF Citation

How to cite this article

Rizwana Anwar, Shahid Karim and S. Riazuddin, 1999. Entomocidal Activity of Bacillus Thuringiensis Transgenic Rice Plants Against Rice Leaffolder, Cnaphalocrocis Medinalis (Lepidoptera: Pyralidae). Pakistan Journal of Biological Sciences, 2: 1472-1477.

DOI: 10.3923/pjbs.1999.1472.1477

URL: https://scialert.net/abstract/?doi=pjbs.1999.1472.1477

Related Articles

Natural Variation among Rice Yellow Stem Borer, Scirpophaga incertulas (Walker) and Rice Leaffolder, Cnaphalocrocis medinalis (Guenee) Populations to Bacillus thuringiensis δ-endotoxins
Occurrence of cry Genes in Bacillus thuringiensis (Bt) Isolates Recovered from Phylloplanes of Crops Growing in the New Delhi Region of India and Toxicity Towards Diamond-back Moth (Plutella xylostella)
Screening of Bacillus thuringiensis Isolates Recovered from Diverse Habitats in India for the Presence of cry1A-type Genes and Cloning of a cry1Ac33 Gene Toxic to Helicoverpa armigera (American Bollworm)

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved