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Pakistan Journal of Biological Sciences
  Year: 1998 | Volume: 1 | Issue: 1 | Page No.: 11-14
DOI: 10.3923/pjbs.1998.11.14
 
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Introgression of G. barbadense genes into G. hirsutum through DNA-mediated Embryo Transformation approach

M. Aslam, M. T. Elahi and N. Iqbal

Abstract:
Attempts were made to incorporate intraploid interpsecific gene(s) from Gossyium barbadense into G. hirsutum through DNA-mediated embryo transformation approach. G. barbadense DNA was injected into the styles/ovaries of G. hirsutum, 24 hours after self pollination. Two phenotypic types of plants i.e. transformed and G. hirsutum like were observed in D population. The transformed plants had clearcut variations in boll weight, yield and other quality traits confirmed to recipient parent. D transformations were also heritable and were transmitted to D generation. The transformed plants had better boll weight (4.9 gm), better fibre fineness (3.9 μg/in) and better fibre strength (101.0 TPPSI) compared to the boll weight (3.0 gms), fibre fineness (4.5 μg/in) and fibre strength (91.0 TPPSI) of G. hirsutum. The transformation efficiency for quantitative traits was about 20 % where as low for qualitative traits like flower petals with red spot (0.08 %). In the present studies transformed genotypes illustrated higher yield potential and better fibre quality than the recipient (G. hirsutum) parent.
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How to cite this article:

M. Aslam, M. T. Elahi and N. Iqbal , 1998. Introgression of G. barbadense genes into G. hirsutum through DNA-mediated Embryo Transformation approach. Pakistan Journal of Biological Sciences, 1: 11-14.

DOI: 10.3923/pjbs.1998.11.14

URL: https://scialert.net/abstract/?doi=pjbs.1998.11.14

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