Submit Manuscript

Pharmacologia

Year: 2018 | Volume: 9 | Issue: 4 | Page No.: 157-168
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 393

Search

Authors

Lin Zhang

Narsimha Reddy

Narsimha Reddy

Cheang Soo Khoo

Sundar Rao Koyyalamudi

Sundar Rao Koyyalamudi

Christopher E. Jones

Keywords

Background and Objective: Lobelia chinensis Lour is an important anticancer herb used in traditional Chinese medicine. Many botanical polysaccharides are known to exhibit immunomodulatory and anticancer activities. This research aimed to analyze the L. chinensis polysaccharides (LCPs) for their biological activities relevant to their anticancer function. Materials and Methods: Water-soluble LCPs were extracted and purified using size-exclusion chromatography to obtain two dominant polysaccharides, LCP-1 and LCP-2 having molecular masses of 1899 kDa and 5.3 kDa, respectively. The antioxidant potentials of the isolated polysaccharides were evaluated by measuring radical scavenging activities against DPPH∙ (2,2-diphenyl-1-picrylhydrazyl radical), ABTS∙+(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical) and OH∙ (hydroxyl radical). Immunostimulatory activities of LCP-1 and LCP-2 were measured using mouse macrophages. Structure of the most active fraction (LCP-2) was determined using FT-IR and NMR spectroscopic techniques. Results: Two isolated polysaccharide fractions displayed significant antioxidant activities and stimulated the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), although LCP-2 is more effective. Detailed structural characterization by FT-IR and NMR was undertaken for the most active fraction (LCP-2) and confirmed that LCP-2 is(2,1)-β-fructan. Conclusion: The results suggested that the polysaccharides isolated from Lobelia chinensis Lour are potential candidates for immune-chemotherapy and suitable for the treatment of cancer.
PDF Fulltext XML References Citation

How to cite this article

Lin Zhang, Narsimha Reddy, Cheang Soo Khoo, Sundar Rao Koyyalamudi and Christopher E. Jones, 2018. Antioxidant and Immunomodulatory Activities and Structural Characterization of Polysaccharides Isolated from Lobelia chinensis Lour. Pharmacologia, 9: 157-168.

URL: https://scialert.net/abstract/?doi=pharmacologia.2018.157.168

Leave a Comment

Your email address will not be published. Required fields are marked *